A fusion tag, called 3 × Flag-tag and consisting of eight amino acids (Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile- Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography.

A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography.

A fusion tag, called HA-tag and consisting of 9 amino acids (YPYDVPDYA) , was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions.

Flag（DYKDDDDK ）标签是由 8 个氨基酸组成的短肽，亲水性强，不会占据其 他表位与结合域，因此更易与抗体结合， Anti-DYKDDDDK Antibody 为小鼠 lgG2B 重组单克隆抗体，能特异性识别融合蛋白 N 端、C 端的 DYKDDDDK 标签， 广泛用于融合蛋白的 纯化、检测和鉴定。

c-Myc 蛋白含有 bHLH 结构域，该蛋白在正常机体发育和肿瘤发生过程中起着 非常重要的作用，c-Myc 是一个重要的转录因子, 其异常表达能促进多种癌 症的的发生和发展。将人 p62 c-Myc 蛋白的 410-419 这段氨基酸序列 （EQKLISEEDL）融合至目的蛋白的 N 端或 者是 C 端，可以对目的蛋白进行准 确的检测、定位和纯化。